Systemic mastocytosis (SM) is a rare clonal hematologic disorder characterized by the abnormal proliferation and accumulation of mast cells in various tissues, including the bone marrow, liver, spleen, gastrointestinal tract, and skin. A key driver of SM is the KIT D816V mutation, found in over 90–95% of adult cases. The KIT D816V mutation drives uncontrolled mast cell growth by altering a key regulatory protein, making it a vital target for SM research. The superRCA® KIT D816V Mutation Kit RUO is a specialized assay designed for quantitative detection of mutation allele frequency in purified DNA samples carrying the KIT D816V variant. Developed for research use only, this kit enables precise measurement of mutant allele frequency (MAF) using flow cytometry, presenting results as a ratio between wild-type and mutant nucleic acid sequences. This assay is optimized for use with the superRCA® Accessory Kit RUO, which provides the necessary enzymes and buffers to support the mutation detection workflow.

* superRCA® is a registered trademark of Rarity Bioscience AB